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DNA Sequencing Laboratory

eagle-i ID

http://msm.eagle-i.net/i/0000012e-5936-2dc1-0e5d-6ffe80000000

Resource Type

  1. Core Laboratory

Properties

  1. Resource Description
    The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab. Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers, Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5) Service Charges: DNA Sequencing: $5/sequencing reaction (MSM), (reaction and electrophoresis) $6/reaction (non-MSM) Large-user sequencing discount $4/reaction (MSM) (full 96-well plate) $5/reaction (non-MSM) DNA Fragment Analysis: $1.50 per sample (MSM) (electrophoresis only) $2 per sample (non-MSM)
  2. Contact
    Roth, William
  3. Contact
    Yang, Qi
  4. Affiliation
    Gene Profiling Core
  5. Performs Technique
    DNA sequencing assay
  6. Website(s)
    http://www.msm.edu/research/research_centersandinstitutes/RCMI/coreFacilities/geneprofiling/dnasequencing.aspx
  7. Website(s)
    https://rcenterportal.msm.edu/node/38
  8. Director
    Roth, William
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    wroth (William Roth)
  3. created
    2011-02-24T13:54:28.080-06:00
  4. creator
    ppullen (Paula Pullen)
  5. modified
    2014-04-25T13:24:19.464-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016