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DNA Sequencing Laboratory

eagle-i ID


Resource Type

  1. Core Laboratory


  1. Resource Description
    The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab. Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers, Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5) Service Charges: DNA Sequencing: $5/sequencing reaction (MSM), (reaction and electrophoresis) $6/reaction (non-MSM) Large-user sequencing discount $4/reaction (MSM) (full 96-well plate) $5/reaction (non-MSM) DNA Fragment Analysis: $1.50 per sample (MSM) (electrophoresis only) $2 per sample (non-MSM)
  2. Contact
    Roth, William
  3. Contact
    Yang, Qi
  4. Affiliation
    Gene Profiling Core
  5. Performs Technique
    DNA sequencing assay
  6. Website(s)
  7. Website(s)
  8. Director
    Roth, William
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    wroth (William Roth)
  3. created
  4. creator
    ppullen (Paula Pullen)
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016