DNA Sequencing Laboratory

eagle-i ID

Resource Type

Properties

1. Resource Description

   The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab. Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers, Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5) Service Charges: DNA Sequencing: $5/sequencing reaction (MSM), (reaction and electrophoresis) $6/reaction (non-MSM) Large-user sequencing discount $4/reaction (MSM) (full 96-well plate) $5/reaction (non-MSM) DNA Fragment Analysis: $1.50 per sample (MSM) (electrophoresis only) $2 per sample (non-MSM)

2. Contact

   Roth, William

3. Contact

   Yang, Qi

4. Affiliation

   Gene Profiling Core

5. Performs Technique

   DNA sequencing assay

6. Website(s)

   http://www.msm.edu/research/research_centersandinstitutes/RCMI/coreFacilities/geneprofiling/dnasequencing.aspx

7. Website(s)

   https://rcenterportal.msm.edu/node/38

8. Director

   Roth, William