eagle-i Morehouse School of MedicineMorehouse School of Medicine

Core Laboratories at Morehouse School of Medicine

This is a summary list of all core laboratories at Morehouse School of Medicine . The list includes links to more detailed information, which may also be found using the eagle-i search app.

Analytical Chemistry & Protein Profiling Core

Summary:

The Analytical Chemistry and Protein Profiling Core is comprised of three service core labs:

A. Analytical Chemistry Lab

The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers. These services include:

1. Custom sample analysis by High-performance liquid chromatography (HPLC).
2. ELISA
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology

B. Luminex Lab

The Luminex Lab has a Bio-Plex 200 multiplex plate reader, which allows simultaneous analytical measurement of multiple protein biomarkers linked to beads, and provides for exquisite sensitivity, specificity, and precise measurements from small sample volumes.

Services include:
1. Multiplex Immunoassays
2. QuantiGene Multiplex Assays

C. Proteomics Lab

Proteins can be identified by trypsin digestion and subsequent analysis of peptides by LC/MS/MS. Samples can be bands or spots from 1D or 2D gel electrophoresis or solutions of moderate complexity. Expression analysis can be done using ProteoIQ software to compare samples. Pathway analysis can be done in order to identify biological processes and relationships. Analysis of protein translational modifications PTMs can also be done.



Analytical Chemistry Laboratory

Summary:

The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers. These services include:

1. Custom sample analysis by High-performance liquid chromatography (HPLC).
2. ELISA
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology

Equipment/Technology

1. High Performance Liquid Chromatography (HPLC): Custom analyses by HPLC can include (but not limited to): amino acids; thiols (total, oxidized, reduced); chiral and racemic compounds such as warfarin and clenbuterol; etc.
i. Agilent 1100 series HPLC equipped with a diode array detector (DAD), variable wavelength detector (VWD), 1046 series fluorescence detector.
ii. Agilent 1100 series HPLC system equipped with 1100 series fluorescence detector.
2. Molecular Devices SpectroMax Plus microtiter plate reader and variable wavelength UV-Visible spectrophotometer to perform Colorimetric and Enzyme-linked Immunosorbent assays.
3. NOVA Electrolytes Analyzer for analyses of Na+, P+, TCa+, Li+, Cl- in biological fluids.

Service Charges

The Analytical Chemistry Laboratory performs custom testing and has a standard fee schedule for services. The fee structure is based on the amount of time required to process the samples (extraction, derivatization, etc.) and the cost of supplies and reagents. For non-NIH sponsored projects (MSM and outside institutions and corporations), labor costs are assessed. A complete listing of currently available tests or cost per requested assay can be emailed or faxed upon request. Additionally, since the Analytical Chemistry Laboratory performs custom testing, if the requested assay is not on the list, please contact the core leader directly for test availability and the most up-to-date pricing.



BIONUTRITION CORE

Summary:

Mission
The Bionutrition Core is committed to reducing health disparities in at risk ethnic minority populations and the underserved through the pursuit of excellence in nutrition research in both clinical and community settings. The core aims to:

• Support investigators conducting nutrition interventions by providing assistance with study development; assessments for dietary intake, body composition and energy expenditure; menus for diet studies, study implementation and compliance monitoring; dietary data analysis and management of core generated data
• Increase the number of investigators conducting nutrition research at MSM
• Provide a training environment to foster an increase in leadership in nutrition research from diverse populations
• Collaborate with investigators seeking to conduct diet and lifestyle modification interventions to prevent disease
• Identify group collaborations that foster novel research in the areas of epigenetics, nutrigenomics and metabolomics in ethnic minorities
• Cultivate nutrition research to identify the molecular action of bioactive food components and their use in prevention of disease in minorities and the underserved
• Promote global nutrition research studies and collaborations that focus on identification of dietary risk factors for disease development in migrant ethnic minorities transitioning to westernized diets versus lower rates of diet-related disease in the host country.

Vision
The Bionutrition Core supports the Morehouse School of Medicine mission to improve the health and well-being of individuals and communities with emphasis on people of color and the underserved. The core is committed to increasing the diversity of professionals in healthcare and science through the promotion of nutrition education, leadership and research necessary to understand and address the unequal burden of health disparities in ethnic minorities.

Services
The Bionutrition Core is comprised of a Metabolic Research Kitchen and Body Composition Lab and provides the following services:
• Nutrition Assessment and Diet History conducted through the use of Food Records, Food Frequency Questionnaires and Composite Analysis.

• Body Composition Assessments performed utilizing Dual Energy X-ray Absorptiometry (DEXA), Anthropometry (skinfolds/circumferences), and Bioimpendance Analysis.

• Resting Energy Expenditure determined via Indirect Calorimetry

• Research Diet Design and Meals for Diet Intervention Studies

• Training for Medical Students and Dietetic Interns

• Protocol Development and Curriculum Design

• Study Coordination, CRF Development and Manual of Operation Design

• Dietary Data Analysis and Management

• Nutrient Analysis programs: Food Processor SQL, NDSR

• Breath Testing for Diet and GI Related Disease



Biological Manipulation Core

Summary:

The Biological Manipulation Core offers model systems for manipulation and analysis of gene expression. Laboratories within this core include:

A. Microvesicle Lab

This Lab offers technical expertise in isolation and purification of Microvesicles, and in their analysis. This includes expertise in Microvesicle isolation and analysis. Further, this group has developed technology that allows targeted Loading of proteins into micro-vesicles. Microparticles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience. This research area at MSM was originally funded through the HIV group, but quickly was turned into a collaborative, transdisciplinary funded research project through the RCMI T1 ARRA Project which brought together diverse researchers from AIDS/ID, Cancer, Cardiovascular/Metabolic focus areas together into a interacting group. This research area can be a collaborative, transdiciplinary research driver at MSM and across the RTRN.

B. Stem Cell Lab

The Stem Cell Lab offers consultative service to investigators interested in adult or embryonic stem cell research including induced pluripotent stem cells (iPSCs) from human and various animals. In addition, the lab offers technical expertise for investigators interested in generating preliminary data for grant application or who are funded to perform stem cell work and who can provide reagents. This includes deriving and growing stem cells from bone marrow, adipose tissue, and umbilical cord, growing commercially available adult embryonic stem cells and iPSCs, reprogramming adult tissue cells into pluripotent cells, and differentiating stem cells into various lineages. The lab is constantly developing new techniques that will benefit the research community. Currently, the focus is on establishing techniques for genome editing in iPSCs with the goal of generating human models of human genetic diseases for translation research.

C. Zebrafish Lab

The Zebrafish Lab offers the combination of low cost, rapid in vivo analysis and complex vertebrate biology. Zebrafish are closer to humans evolutionarily than yeast or insects or worms and experiments are faster and less costly than those using mice. This model system supports research in many research areas, including molecular genetics of cardiac development, heart disease, neurogenetics, cell signaling pathways, and genetics of cancer prevention, and is an excellent teaching tool to support research training programs.

The Zebrafish Lab will provide consultative and fee for services to basic, translational and clinical investigators; for example, animal husbandry, embryo DNA microinjection, generation of transgenic models, morpholino knockdown, in vivo chemical screening strategies, and other custom services, as needed. All services are charged on an hour-by-hour consultation basis. For more information, please contact the core leader directly.



Biomedical Informatics Unit (BIU)

Summary:

The Biomedical Informatics Unit (BIU) of the Morehouse School of Medicine (MSM) RCMI Center of Excellence for Clinical and Translational Research (R- CENTER) focuses on integration of MSM and affiliated health information systems -- electronic medical and personal health records, patient databases, and other research databases (basic, translational, clinical and population-based) -- into an interoperable repository. The long-term goal is to establish an integrated standards-based informatics platform to support MSM clinical and translational research investigators in their own work and in sharing research information with collaborators and other investigators in parallel fields. Ultimately, the interoperable repository platform will expand scientific discovery and research translation of MSM investigators in collaboration with A-CTSI partners as well as the RCMI consortium. The building blocks of the repository have begun with the i2b2 platform.

i2b2 (Informatics for Integrating Biology and the Bedside) is an NIH-funded National Center for Biomedical Computing based at Partners HealthCare System. The i2b2 is a scalable informatics framework that will bridge clinical research data and the vast data banks arising from basic science research in order to better understand the genetic bases of complex diseases. This knowledge will facilitate the design of targeted therapies for individual patients with diseases having genetic origins. i2b2 is being implemented across a number of collaborative research networks. These linked data will serve as a major institutional resource for advancing clinical/translational research, and enhancing career development for clinical/translational research, community health, health services/outcomes and clinical trials. The creation of an integrated data repository will greatly enhance the training and career development goals of the R-CENTER by facilitating ready access to critical research data that will support trainees in the CRECD program, junior investigators, pilot grant awardees and other researchers across the MSM R-CENTER.

The Bioinformatics Lab facilitates analysis of gene expression data gathered from multiplatform microarrays (Agilent, Affymetrix, etc) as well as provides analysis and consultation related to various expression profiling technologies which include RNA, miRNA, ChIP-on-Chip, and Antibody microarrays and post-hoc analysis of SNP and SAGE data.



Biomedical Technology Service Core Laboratory

Summary:

The Biomedical Technology Service Laboratory (BTSL) Core facility has been in existence for more than twenty years. The BTSL operates in multiple locations which house basic, multi-use equipment, available to all researchers, including: autoclaves, centrifuges, incubators for bacteria and cell cultures, spectrophotometers, scintillation counters, plate readers, luminometer, sonicators and microscopes. The BTSL also provides liquid nitrogen, dry and wet ice, glassware washers and laundry facilities.



Cell and Tissue Imaging Core

Summary:

"The Cell and Tissue Imaging Facility provides a broad range of services covering many of the major structural methods used to study cells and tissues. The Core consists of four labs: Electron Microscopy, Flow Cytometry and Cell Sorting, Image Analysis, and
Molecular Histology."

The Electron Microscopy Lab provides complete scanning and transmission services from specimen fixation and dehydration, to examination and documentation via darkroom photography.

The Flow CytometryFACS, and Magnetic-bead Cell-sorting Lab houses a BD FACS Aria II and Miltenyi Biotec autoMACS Pro Seperator. Certfied BD- and Miltenyi-trained staff are available for consultation, acquiring data, or sorting cells.

The Image Analysis Lab provides a wide variety of options for the analysis and quantification of fluorescent probes, markers and antibodies within fixed and living cells. This lab houses a Leica TCS SP5 Confocal microscope, A DeltaVision Core Deconvolution microscope, a Tecan plate reader and a Zeiss Axioplan upright fluorescent microscope.

The Molecular Histology Lab is equipped to provide the following services: paraffin embedding, paraffin sections, frozen sections, immunohisto-chemical and H&E staining, and in situ hybridization."

For more information and service charges, contact:
Dr. James Lillard
Flow Cytometry and Cell Sorting Core Leader
Email: jlillard@msm.edu
Phone: (404) 752-1863

Dr. Woo-Kuen Lo
Electron Microscopy Core Leader
Email: wlo@msm.edu
Phone: (404) 752-1558

Dr. John Patrickson
Molecular Histology Core Leader
Email: jpatrickson@msm.edu
Phone: (404) 752-5717

Dr. Xuebiao Yao
Confocal & Fluorescent Imaging Core Leader
Email: xyao@msm.edu
Phone: (404) 752-1894



Center of Laboratory Animal Resources

Summary:

Access to CLAR facilities is restricted to authorized investigators with CLAR and IACUC approval. No individuals may enter the facilities without this CLAR/IACUC authorization.

Since its inception, the Center for Laboratory Animal Resources (CLAR) has centralized most research and teaching animal care for the Atlanta University Center consortium. Our purpose is to provide total animal care services as needed to all academic and/or research faculty and staff affiliated with any one of the member institutions of the Atlanta University Center (AUC).



DNA Sequencing Laboratory

Summary:

The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab.

Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers,

Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5)

Service Charges:
DNA Sequencing: $5/sequencing reaction (MSM),
(reaction and electrophoresis) $6/reaction (non-MSM)
Large-user sequencing discount $4/reaction (MSM)
(full 96-well plate) $5/reaction (non-MSM)
DNA Fragment Analysis: $1.50 per sample (MSM)
(electrophoresis only) $2 per sample (non-MSM)



Electron Microscopy Laboratory

Summary:

Established in 1985, the RCMI Electron Microscopy Core facility provides complete scanning and transmission services, from specimen fixation/dehydration/critical point drying/ embedding/polymerization/ block trimming/sectioning/slide-making/formvar-coating, to examination and documentation of prepared samples utilizing JEOL 820 scanning and JEOL 1200EX transmission electron microscopes.



Flow Cytometry & Cell Sorting Laboratory

Summary:

The Cell and Tissue Imaging Facility provides a broad range of services covering many of the major structural methods used to study cells and tissues. The Core consists of four labs: histological and microscopy training for graduate students and tissue processing and collection services for the research faculty at MSM and other institutions. This facility provides a broad range of services covering many of the major structural methods used to study cells and tissues.



Functional Genomics Core Laboratory

Summary:

The Morehouse School of Medicine Functional Genomics Core is an RCMI funded core that provides fee-based microarray ‘-OMICS’ services for research investigators using glass slide technology platforms. We are an Agilent Certified Core facility and a member of the Association of Biomolecular Research Core Facilities (ABRF) worldwide. Specifically we provide assistance, or full services, from RNA isolation to scanning hybridized microarrays and raw data extraction. In conjunction with the RCMI Bioinformatics Core, we provide full services in the analyses of ‘-omics’ datasets. We support all microarray products from Agilent as well as any other array company that utilizes uncoated glass slide technologies. We also provide consultative services for custom microarray design.



Gene Profiling Core

Summary:

The RCMI Gene Profiling Core provides a variety of services related to analysis of gene expression, which includes genome and transcriptome analysis. Laboratories in the Gene Profiling Core include Functional Genomics, Gene Variation and DNA Sequencing labs.

The DNA Sequencing Lab provides both DNA sequence and fragment analysis of a variety of DNA templates, including genomic DNA, PCR amplicons and plasmid or cosmid constructs. The lab uses capillary electrophoresis sequencing technology and is built around the ABI 3130xl Gene Analyzer (16-capillary) and associated software for DNA sequence analysis.

The Functional Genomics Lab provides microarray services for research investigators using glass slide technology platforms. We are an Agilent Certified Core facility and a member of the Association of Biomolecular Research Core Facilities (ABRF). Specifically we provide assistance, or full services, from RNA isolation to scanning hybridized microarrays and raw data extraction. In conjunction with the Biomedical Informatics Unit (BIU) at MSM, we provide full services in the analyses of ‘-omics’ datasets. We support all microarray products from Agilent as well as any other array company that utilizes uncoated glass slide technologies. We also provide consultative services for custom microarray design.

The Gene Variation Core Lab assists investigators in application of genomic technologies to population-based research. The strategic plan is to create a link between basic science, clinical investigation and population-based research. The central hypothesis is that health disparities are related in part to ethnicity-specific DNA variants in critical genes that influence the susceptibility to common diseases. Accordingly, the major objective of the lab is to enhance health disparities research capacity at the molecular genetic level based on cutting-edge genomic technologies.

For more information about Gene Profiling Core services contact the individuals listed here or click on the links to the affiliated labs.

Nerimiah Emmett. PhD
Functional Genomics Core Leader
Medical Education Building C-346
Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310-1495
Telephone 404-752-1679
nemmett@msm.edu

William Roth, PhD
DNA Sequencing Core Leader
Hugh Gloster B-315C
Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310-1495
Telephone 404-752-1947
wroth@msm.edu


Qing Song, PhD
qsong@msm.edu
Gene Variation Core Leader
Research Wing D-203
720 Westview Drive
Atlanta, GA 30310
Telephone 404-752-1845



Gene Variation Core Laboratory

Summary:

The Human Genotyping Lab assists investigators to apply the genomic technologies to population-based research. The strategic plan is to create a link between basic science, clinical investigation and population-based research by integrating genomic sciences into our research program. The central hypothesis is that health disparities are related in part to ethnicity-specific DNA variants in critical genes that influence the susceptibility to common diseases. Morehouse School of Medicine has a longstanding interest on the studies of ethnic disparities and a longstanding strength on community outreach to underserved minority populations. Accordingly, the major objective of the MSM Human Genome Core Laboratory is to enhance the research capacity on these sample cohorts and upgrade our research to the molecular genetic level based on cutting-edge genomic technologies.



Image Analysis Core Laboratory

Summary:

The facility provides a wide variety of options for the analysis and quantification of fluorescent probes, markers and antibodies within fixed and living cells.



Luminex Laboratory

Summary:

The Luminex Laboratory has Bio-Plex 200 and MagPix multiplex plate readers. These instruments allow simultaneous analytical measurement of multiple protein biomarkers using bead-linked assays. This provides for exquisite sensitivity, specificity, and precise measurements of analytes in small sample volumes.

The lab also offers 2D Gel services. The lab houses a BioRad automated spot picker (Proteome Works) and a BioRad gel imager (Molecular imagerFX). Bundled with this package is the latest version of PDQuest 8.0 for analysis of expression data from gels. Together this equipment allows us to offer services such as protein identification and analysis of protein modifications. This service complements LC/MS/MS analysis offered in the roteomics Lab.

Services include:
1. Multiplex Immunoassays
2. QuantiGene Multiplex Assays
3. 2D Gel analysis

Equipment/Technology
1. Luminex Bio-Plex 200, and MagPix readers
2. Bio-Plex Manager 6.0
3. BioRad 2D gel insruments
4. PD Quest 8.0

Service Fee
$ 200.00 per assay plate (Bioplex)
2D Gel Service by consultation



Microvesicle Core Laboratory Facility

Summary:

This Lab offers technical expertise in isolation and purification of Microvesicles, and in their analysis. Technical Expertise: Dr. Ming Bo Huang, M.D. has formulated expertise through ongoing research in the MSM HIV Nef Pathogenesis Group. Dr. Huang has developed expertise in Microvesicle isolation and analysis. Further, this group has developed technology that allows targeted Loading of proteins into micro-vesicles. Microparticles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience. This research area at MSM was originally funded through the HIV group, but quickly was turned into a collaborative, transdisciplinary funded research project through the RCMI T1 ARRA Project which brought together diverse researchers from AIDS/ID, Cancer, Cardiovascular/Metabolic focus areas together into a interacting group. This research area can be a collaborative, transdiciplinary research driver at MSM and across the RTRN.



Molecular Histology Core Laboratory

Summary:

This facility is equipped to provide basic histological services including: paraffin embedding, paraffin sections, frozen sections, immunohistochemical and H&E staining, and in situ hybridization. The facility consists of a paraffin tissue embedding center, rotary microtomes, cryostat, tissue floatation bath, microwave, tissue processor, and slide warmers.



Proteomics Laboratory

Summary:

Proteins can be identified by trypsin digestion and analysis of peptides by LC/MS/MS. Samples can be bands or spots from 1D or 2D gel electrophoresis or solutions of moderate complexity. Expression analysis can be done using ProteoIQ software to compare samples. Pathways analysis can be done to identifity biological processes and relationships. Analysis of protein translational modifications PTMs can also be done.



Stem Cell Core Laboratory

Summary:

The Stem Cell Lab offers consultative service to investigators interested in adult or embryonic stem cell research including induced pluripotent stem cells (iPSCs) from human and various animals. In addition, the lab offers technical expertise for
investigators interested in generating preliminary data for grant application or who are funded to perform stem cell work and who can provide reagents. This includes deriving and growing stem cells from bone marrow, adipose tissue, and umbilical cord, growing commercially available adult embryonic stem cells and iPSCs, reprogramming adult tissue cells into pluripotent cells, and differentiating stem cells into various lineages. The lab is constantly developing new techniques that will benefit the research community. Currently, the focus is on establishing techniques for genome editing in iPSCs with the goal of generating human models of human genetic diseases for translation research.



Study Design, Biostatistics, and Data Management Core (SDBDMC)

Summary:

The specific objectives of the Study Design, Biostatistics, and Data Management Core are:

1: To provide consultation in study conceptualization and design, sampling, and sample size planning, statistical analyses planning, statistical analyses methodologies, and epidemiologic principles and methods.
2: To provide biostatistical analysis and data analytical support
3: To provide technical support and consultation in data collection methods, data entry, processing, and management.
4: To provide database administration support in creating and managing databases as shared resources

Services provided by the SDBDMC include:
Power and sample size calculations, Statistical analysis, Proposal development, Program evaluation, Evaluation plan development, Manuscript preparation, Study design, Survey development, Methodology development, Focus group moderation, Qualitative analysis, Data collection form development, and Database development and management.

We have streamlined the assistance request process by developing a web-based research assistance request form. The automated process captures more specifically the type of requests by faculty, staff, and students and allows these "customers" throughout MSM's campus and off site locations easier access the SDBDMC. Click the website link below for assistance.



Zebrafish Biology Core Facility

Summary:

The Zebrafish Lab offers the combination of low cost, rapid in vivo analysis and complex vertebrate biology. Zebrafish are closer to humans evolutionarily than yeast or insects or worms and experiments are faster and less costly than those using mice. This model system supports research in many research areas, including molecular genetics of cardiac development, heart disease, neurogenetics, cell signaling pathways, and genetics of cancer prevention, and is an excellent teaching tool to support research training programs.

Service Charges:

The Zebrafish Lab will provide consultative and fee for services to basic, translational and clinical investigators; for example, animal husbandry, embryo DNA microinjection, generation of transgenic models, morpholino knockdown, in vivo chemical screening strategies, and other custom services, as needed. All services are charged on an hour-by-hour consultation basis. For more information, please contact the core leader directly.



Found 22   core laboratories .

This institution proudly participates in the eagle-i Network. Use the search bar above to search more than 90,000 biomedical research resources currently listed in eagle-i from more than 28 institutions. Not sure what you're looking for? Choose "Browse" at the top of the page to see organisms, instruments, reagents, services and more.

To learn more about the eagle-i Network, resources and institutions, please visit www.eagle-i.net.

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016