The Analytical Chemistry and Protein Profiling Core is comprised of three service core labs:
A. Analytical Chemistry Lab
The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers. These services include:
1. Custom sample analysis by High-performance liquid chromatography (HPLC).
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology
B. Luminex Lab
The Luminex Lab has a Bio-Plex 200 multiplex plate reader, which allows simultaneous analytical measurement of multiple protein biomarkers linked to beads, and provides for exquisite sensitivity, specificity, and precise measurements from small sample volumes.
1. Multiplex Immunoassays
2. QuantiGene Multiplex Assays
C. Proteomics Lab
Proteins can be identified by trypsin digestion and subsequent analysis of peptides by LC/MS/MS. Samples can be bands or spots from 1D or 2D gel electrophoresis or solutions of moderate complexity. Expression analysis can be done using ProteoIQ software to compare samples. Pathway analysis can be done in order to identify biological processes and relationships. Analysis of protein translational modifications PTMs can also be done.