Electron Microscopy Laboratory

Director: Lo, Woo-Kuen, Ph.D.

Summary:

Established in 1985, the RCMI Electron Microscopy Core facility provides complete scanning and transmission services, from specimen fixation/dehydration/critical point drying/ embedding/polymerization/ block trimming/sectioning/slide-making/formvar-coating, to examination and documentation of prepared samples utilizing JEOL 820 scanning and JEOL 1200EX transmission electron microscopes.

Affiliations:

• Cell and Tissue Imaging Core
• Flow Cytometry & Cell Sorting Laboratory
• Image Analysis Core Laboratory
• Molecular Histology Core Laboratory
• Research Centers in Minority Institutions (RCMI), Morehouse School Of Medicine

People:

• Director: Lo, Woo-Kuen, Ph.D.
  

Member: Brako, Lawrence
Role: Lab Technician

Resources:

Instruments

• Hummer Sputtering System ( Instrument )
  

  Specimen Coater for Scanning Electron Imaging

• JEOL JEM-1200EX ( Transmission electron microscope )
  

• JEOL JSM-820 ( Scanning electron microscope )
  

• Tousimis Critical Point Dryer ( Critical point dryer )
  

  Equipment for drying biological specimens for Scanning Electron Imaging

• Ultramicrotome RMC MT-7 ( Microtome )
  

  Equipment for cutting and trimming sections from cured resin blocks

Services

• Development & scanning service ( Support service )
  

  Electron microscopy negatives are developed and scanned onto CD for the investigator.

• Examination and documentation service ( Material analysis service )
  

  "Examination and documentation of prepared specimens utilizing Transmission and/or Scanning Electron Microscope."

• Sample preparation for electron microscopy ( Material processing service )
  

  "Complete preparation of samples (i.e. fixation, dehydration, critical point drying, sputter coating, embedding, polymerization, knife-making, trimming, H/E slide-preparation, sectioning, formvar-coated grid preparation and
  subsequent grid-staining)."

Web Links:

• http://www.msm.edu/research/research_centersandinstitutes
  /RCMI/coreFacilities/celltissueimaging/coreElectron.aspx