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DNA Sequencing Laboratory

Director: Roth, William


The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab.

Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers,

Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5)

Service Charges:
DNA Sequencing: $5/sequencing reaction (MSM),
(reaction and electrophoresis) $6/reaction (non-MSM)
Large-user sequencing discount $4/reaction (MSM)
(full 96-well plate) $5/reaction (non-MSM)
DNA Fragment Analysis: $1.50 per sample (MSM)
(electrophoresis only) $2 per sample (non-MSM)





  • ABI GeneAmp 9600 PCR system ( PCR instrument )

  • Applied Biosystems 3130xl Gene Analyzer ( DNA sequencer )

    "Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity."

  • New Brunswick Scientific Innova 4300 ( Incubator shaker )

    Shaker-incubator for growth of bacterial cultures.



  • Applied Biosystems Suite ( Software )

    Suite includes:
    * Sequence Analysis 5.2
    * GeneMapper 4.0
    * SeqScape 2.5
    * Invitrogen Vector NTi10.3 suite
    * Sequencer 4.2
    * Geneious 4.0.2

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Last updated: 2014-04-25T13:24:19.464-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016