The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab.
Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers,
Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5)
DNA Sequencing: $5/sequencing reaction (MSM),
(reaction and electrophoresis) $6/reaction (non-MSM)
Large-user sequencing discount $4/reaction (MSM)
(full 96-well plate) $5/reaction (non-MSM)
DNA Fragment Analysis: $1.50 per sample (MSM)
(electrophoresis only) $2 per sample (non-MSM)
Role: Lab Technician
"Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity."
Shaker-incubator for growth of bacterial cultures.
Includes reaction and capillary electrophoresis.
"Expanded options include enumeration of polymorphisms (SNPs) in patient samples, a service which can be used to complement SNP discovery protocols offered in the Human Genotyping Lab."
* Sequence Analysis 5.2
* GeneMapper 4.0
* SeqScape 2.5
* Invitrogen Vector NTi10.3 suite
* Sequencer 4.2
* Geneious 4.0.2