Molecular Histology Core Laboratory

Director: Patrickson, John

Summary:

This facility is equipped to provide basic histological services including: paraffin embedding, paraffin sections, frozen sections, immunohistochemical and H&E staining, and in situ hybridization. The facility consists of a paraffin tissue embedding center, rotary microtomes, cryostat, tissue floatation bath, microwave, tissue processor, and slide warmers.

Affiliations:

• Cell and Tissue Imaging Core

People:

• Director: Patrickson, John
  
• PI: Bond, Vincent C., Ph.D.
  

Member: Pitts, Sidney
Role: Lab Technician

Resources:

Services

• Frozen sectioning ( Material analysis service )
  

  "The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details)
  
  For frozen tissue preparations contact the laboratory."

• Immunohistochemical and H&E staining ( Material analysis service )
  

  "The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details)
  
  For frozen tissue preparations contact the laboratory."

• In situ hybridization ( Material analysis service )
  

  "The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details)
  
  For frozen tissue preparations contact the laboratory."

• Paraffin embedding ( Material analysis service )
  

  "This facility is equipped to provide basic histological services including: paraffin embedding, paraffin sections, frozen sections, immunohistochemical and H&E staining, and in situ hybridization. The facility consists of a paraffin tissue embedding center, rotary microtomes, cryostat, tissue floatation bath, microwave, tissue processor, and slide warmers."

• Paraffin sectioning ( Material analysis service )
  

  "The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details)
  
  For frozen tissue preparations contact the laboratory."

Web Links:

• http://www.msm.edu/research/research_centersandinstitutes
  /RCMI/coreFacilities/celltissueimaging/coreMolecular.aspx