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|  Morehouse School of Medicine |
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| Morehouse School of Medicine |
The Morehouse School of Medicine Functional Genomics Core is an RCMI funded core that provides fee-based microarray ‘-OMICS’ services for research investigators using glass slide technology platforms. We are an Agilent Certified Core facility and a member of the Association of Biomolecular Research Core Facilities (ABRF) worldwide. Specifically we provide assistance, or full services, from RNA isolation to scanning hybridized microarrays and raw data extraction. In conjunction with the RCMI Bioinformatics Core, we provide full services in the analyses of ‘-omics’ datasets. We support all microarray products from Agilent as well as any other array company that utilizes uncoated glass slide technologies. We also provide consultative services for custom microarray design.
Member:
Leander-Griffith, Michelle V.
Role:
Research Assistant
"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."
"Agilent’s high-resolution microarray scanner is part of an improved microarray platform that provides greater transcript definition and chromosomal definition, as well as expression measurements that more accurately reflect the range of gene activity in biological systems. Agilent’s system produces accurate relative quantitation of signal levels over a range of more than six orders of magnitude, far exceeding the capabilities of other systems. This enhances data quality across a broad range of applications such as gene expression, miRNA profiling, array CGH, ChIP, and methylation assays."
Scanner for Microarrays.
Available as mRNA expression microarrarys.
Available are mRNA expression microarrarys
Available are mRNA expression, Exon, miRNA, Encode chip-on-chip, promotor, CpG Island, DNA Methylation, CNV, CGH, and CGH+SNP microarrarys.
The Agilent Human Genome CGH Microarray is a high-resolution tool for genome-wide DNA copy number variation profiling without amplification or complexity reduction. Comprehensive probe coverage is enhanced with emphasis on known genes, promoters, miRNAs, pseudoautosomal, and telomeric regions.
The Agilent Human DNA Methylation Microarray is designed to interrogate known CpG islands and two additional groups of methylated DNA. The array is specifically designed for analysis of methylated DNA derived from affinity-based isolation methods such as methylated DNA immunoprecipitation (MDIP).
The Agilent Human CpG Island Microarray provides researchers with comprehensive, genome-wide insight into how DNA methylation changes may be correlated to critical biological development processes including cancer, genomic imprinting, gene silencing and chromatin stability. Probes optimized for maximum sensitivity and specificity.
The Human ENCODE ChIP-on-chip Microarray is optimized using probes specifically validated for ChIP-on-chip technology. It is designed to specifically identify human DNA binding proteins within the ENCODE regions through traditional chromatin immunoprecipitation (ChIP) pull-down coupled with powerful DNA microarray location analysis.
The Agilent SurePrint G3 Exon Microarrays make it easy to transition from standard
expression analysis to exon analysis. Probes are designed to target all exons within genes based on well-annotated and up-to-date databases, ensuring both comprehensive and relevant coverage across the genome. Our high-quality 60-mer probes ensure high sensitivity and accuracy for all Agilent gene expression microarrays, including the new exon arrays. The Low Input Quick Amp WT Labeling Kit (LIQAWT) makes amplification and labeling from a low amount of RNA quick and easy. Having been co-developed with these arrays, LIQA WT is optimized to maintain an accurate representation of RNA species, now across whole transcripts. In addition, GeneSpring 11.5 provides new features for the analysis of splicing events along with gene-level expression, lending confidence in the detection of subtle changes now possible with the exon microarrays system.
The Agilent SurePrint G3 Human Comparative Genomic Hybridization (CGH) platform has been extended to include a set of Single Nucleotide Polymorphism (SNP) probes on the same microarray. This allows for the simultaneous, high-resolution detection of copy number and copy-neutral variations, without running two separate microarray experiments. The same straight-forward Agilent CGH workflow is used to prepare the samples for hybridization to the SurePrint G3 Human CGH+SNP microarrays. Restriction digestion of genomic DNA allows genotyping of SNPs located in the enzymes’ recognition sites. After labeling and hybridization, CGH+SNP microarray data are analyzed using novel algorithms contained within Agilent’s Genomic Workbench software.
Agilent SurePrint G3 and High-definition CNV microarrays enable multiple loci to be assessed simultaneously, allowing for their identification and characterization. The microarrays provide high-resolution and optimized probe design for maximal sensitivity and specificity as required by the researcher.
Agilent Human microRNA Microarrays generate complete microRNA profiles in order to provide broad insight into human expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands.
"The Agilent SurePrint G3 Mouse Exon Microarrays come in two options, offering a choice in coverage and throughput. Offering probes targeting individual exons, these arrays make it possible to look beyond the gene to more subtle but relevant expression changes. Agilent Gene Expression Microarrays remain the fast, simple, and affordable choice in gene expression analysis, with new capabilities to drill down into important RNA changes including those at the exon level. Importantly, the gold standard sensitivity provided by Agilent Gene Expression Microarrays has been retained in these new Exon versions, so that even the smallest fold changes are detected."
Available are mRNA expression, Exon, miRNA, promotor, CpG Island, DNA Methylation, CNV, CGH, and CGH microarrarys.
Available are mRNA expression microarrarys.
GenUs BioSystems standard gene expression service includes all materials, processing, quality control, and reporting - including follow-up analysis for a single price. Specialists in small, difficult sample handling we regularly handle sample sizes down to 10 cells (Total RNA yield ~ 100 picograms) utilizing state of the art single and double amplification protocols.
Available are mRNA expression microarrarys.
Available are mRNA expression, Exon, miRNA, promotor, and CGH microarrarys.
Available are mRNA expression microarrarys.
Used for RNA purification.
"The Xenopus Microarray project is supported by the Rockefeller University; it is part of the Laboratory of Molecular Vertebrate Embryology headed by Professor Ali Brivanlou. Software tools and database development are provided by the Laboratory of Computational Genomics headed by Assistant Professor Terry Gaasterland."
Includes mouse whole genome microarrays, human whole genome microarrays, and rat whole genome microarrays.
Available microarray services:
* Hybridization
* Image Quality Control
* Scanning
Service includes:
* miRNA amplification
* miRNA isolation
* miRNA labeling-cyanine dye
* miRNA purification
* miRNA quality assessment
* miRNA quantitation
RNA services offered by the Functional Genomics Core include:
* RNA amplification
* RNA isolation
* RNA labeling –Cyanine dye
* RNA purification
* RNA quality control assessment
* RNA quantitation
We offer RNA isolation from cultured cells and tissues.
We measure the integrity of total RNA samples using the Agilent Bioanalyzer. The Agilent Bioanalyzer uses a micro channel based electrophoretic cell that allows rapid and sensitive investigation of nucleic acids.