This Lab offers technical expertise in isolation and purification of Microvesicles, and in their analysis. Technical Expertise: Dr. Ming Bo Huang, M.D. has formulated expertise through ongoing research in the MSM HIV Nef Pathogenesis Group. Dr. Huang has developed expertise in Microvesicle isolation and analysis. Further, this group has developed technology that allows targeted Loading of proteins into micro-vesicles. Microparticles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience. This research area at MSM was originally funded through the HIV group, but quickly was turned into a collaborative, transdisciplinary funded research project through the RCMI T1 ARRA Project which brought together diverse researchers from AIDS/ID, Cancer, Cardiovascular/Metabolic focus areas together into a interacting group. This research area can be a collaborative, transdiciplinary research driver at MSM and across the RTRN.
1. Beckman Allegra X-15R Tabletop Centrifuge (Rotor: FX6100)
2. Beckman Optima TLX Ultracentrifuge (Rotors: TLA-100.4, TLA-120.2)
3. Beckman Optima Max-XP Ultracentrifuge (Rotors: TLA-100.4, TLA-120.2)
4. Beckman L8M-80 Ultracentrifuge (Rotors: 50.2Ti, 42.1 Ti)
Nanosight LM10-HSBF Nanoparticle Characterization System.
I. Basic Principles
Within a specially designed and constructed laser illumination device mounted under a microscope objective, particles in the liquid sample which pass through a beam path are seen by the instrument as small points of light moving rapidly under Brownian motion. The Microvesicle core Nanosight has the visual laser and measures (i) particle/vesicle size, and (ii) particle/vesicle number.
II. Nanoparticle Tracking Analysis (NTA) applied to the study of microvesicles
Microvesicles (100nm-1µm diameter) and exosomes (30 to 100 nm diameter) are small membrane vesicles secreted by most cell types, e.g. normal, diseased, and neoplastic cells and are present in blood and other bodily fluids. They contain a variety of molecules including signal peptides, mRNA, microRNA. It is significance in the development of vaccines, in targeting tumors, and in diagnosis and/or early detection. These microvesicles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience.
1. Isolation and Purification Microparticle or Exosome from Cell Cultures.
2. Isolation and Purification Microparticle or Exosome from Plasma.
3. Isolation and Purification Micropartical or Exosome from Urine.
4. Microparticle or Exosome samples
1. Microparticle or exosomes pictures (including 3D intensity image)
2. Microparticle or exosomes video (10-90 seconds/sample)
3. Microparticle or exosomes numbers (particle/ml)
4. Microparticle or exosomes size (10nM-1µM)